Institute of Biochemistry

Sukurta: 15 September 2013

bchi 12 Mokslininkų, LT-08662 Vilnius
Tel. 272 9144, fax 272 9196
E-mail:
www http://www.bchi.vu.lt

Director - Prof. Habil. Dr. Valdas Stanislovas Laurinavičius

STAFF

57 research fellows (5 habilitated doctors, 46 doctors), 19 doctoral students.

Department of Bioanalysis
Department of Bioelectrochemistry and Biospectroscopy
Department of Bioorganic Compounds Chemistry
Department of Xenobiotics Biochemistry
Department of Molecular Microbiology and Biotechnology
Proteomics Centre
Department of Molecular Cell Biology
Vivarium
Department of Scientific Information

MAIN RESEARCH AREAS OF THE INSTITUTE

Signaling pathways and epigenetic regulation in cancer and stem cells
Investigation of the structure and function of biocatalyzers
Study of the structure and function of self-assembled lipid systems and membrane proteins

DEPARTMENT OF BIOANALYSIS

Head – Prof. Habil. Dr. Valdas Stanislovas Laurinavičius

STAFF

Chief research fellows: Prof. Habil. Dr. V. Laurinavičius, Prof. Habil Dr. V. Razumas, Prof. Habil. Dr. J. Kulys.
Senior research fellows: Dr. J. Razumienė, Dr. J. Barauskas, Dr. R. Šimkus, Dr. R. Vidžiūnaitė.
Research fellows: Dr. B. Kurtinaitienė, Dr. L. Tetianec.
Junior research fellow: I. Bratkovskaja.
Senior assistant: Dr. M. Dagys.
Doctoral students: E. Voitechovič, M. Jankunec, E. Latynis, A. Laurytėnas, A. Chaleckaja, I. Sakinyte.

RESEARCH INTERESTS

Investigation of enzyme structure and functions, investigations of redox substrates and mediators
Development and investigation of bioelectrocatalytical systems for biosensors and rectors
Investigation of biomolecules in self-organized colloidal systems, investigation of interactions between biomolecules and lipid matrices
Bioluminescence imaging as a tool for the analysis of the cell transport properties
Mathematical modelling of heterogeneous bioelectrocatalytic systems

RESEARCH PROJECTS CARRIED OUT IN 2012

Project Supported by University Budget

Creation and Investigation of Bioelectrocatalytical Systems Based on PQQ-Dehydrogenases and Fungal Laccases. Mathematical Modeling of Bio-Catalytical Systems. Prof. V. Laurinavičius. 2011–2016.

New organic compounds as mediators and polymers as supports for enzyme immobilization have been investigated and applied for the bioelectroanalysis of biologicaly important compounds in carbon-based electrochemical systems. Bioconversion rate and efficiency of the glycerol-3-phosphate in reactor containing immobilized glycerol phosphate oxidase were investigated. Mathematical models of few heterogeneous bioelectrocatalytic systems have been created.
The kinetics of oxidation of artificial substrates catalyzed by novel fungal laccases was investigated. The influence of buffer solution components, pH and inhibitor action of halides on the laccases activity was evaluated. The function of the laccases on gold electrodes containing naked gold nanoparticles as well as modified by specific thiol compounds were analyzed.
Small angle X-ray diffraction, resonance Raman spectroscopy with 413 nm excitation, and non-resonance Raman technique with 785 nm excitation were used to study the influence of entrapped cytochrome c on the structure of hydrated phytantriol liquid-crystalline phases as well as conformational changes of heme group and secondary structure of the protein.
The self-organization of bioluminescent E. coli in suspensions was investigated. The experimental and numerical results showed that luminous bacteria form a weak gel near interfaces.

Projects, Programs, Issues Supported by Research Council of Lithuania

Molecular Mechanisms of Toxicity and Antitumor Activity of Quinones and Polyphenols: Enzymatic Redox Reactions, Cytotoxicity, Signal Transduction, and Proteomics. Habil. Dr. N. Čėnas, 2011–2015.

Bee Products Enriched with Plant Components, the Composition and Properties. Dr. B. Kurtinaitienė. 201–-2015.

Projects supported by Agency for Science, Innovation and Technology (MITA)

Search of New and Genetically Modified Oxidoreductases for Creation of Biofuel Cells (BIOFUELCELL), common project with JSC „Lithuania Research Center“. Project coordinator – Dr. M. Dagys.

International Science Programmes and Projects

COST Action CM0701: Cascade Chemoenzymatic Processes – New Synergies between Chemistry and Biochemistry (CASCAT). Dr. J. Razumiene. 2008–2012.

The European Social Fund under National Integrated Programme Biotechnology & Biopharmacy, grant VP1-3.1-SMM- 08-K01-005. (J. Razumiene the head of Action 1.1.4.)

The European Social Fund under Measure VP1-3.1-ŠMM-07-K “Support to Research of Scientists and Other Researchers (Global Grant)”, Project Developing Computational Techniques, Algorithms and Tools for Efficient Simulation and Optimization of Biosensors of Complex Geometry. (J. Razumienė the participant).

EC Leonardo Da Vinci – Transfer of Innovation Food Legislation, Impact Analysis, Training and Cooperation Network in Europe-E-Learning (FoodIndustry). Prof. V. Laurinavičius. 2012– 2014.

MAIN PUBLICATIONS

Articles

L. Marcinkevičienė, J. Stankevičiūtė, I. Bachmatova, R. Vidžiūnaitė, A. Chaleckaja, R. Meškys. Biocatalytic properties of quinohemoprotein alcohol dehydrogenase IIG from Pseudomonas putida HK5 // Chemija / Lietuvos mokslų akademija. Vilnius : Lietuvos mokslų akademijos leidykla. 2012, t. 23, nr. 3, p. 223-232.

D. Chang, M. Jankunec, J. Barauskas, F. Tiberg, T. Nylander. Adsorption of lipid liquid crystalline nanoparticles: effects of particle composition, internal structure, and phase behavior // Langmuir. Washington : American Chemical Society. 2012, vol. 28, iss. 29, p. 10688-10696.

D. Chang, M. Jankunec, J. Barauskas, F. Tiberg, T. Nylander. Adsorption of lipid liquid crystalline nanoparticles on cationic, hydrophilic, and hydrophobic surfaces // ACS applied materials and interfaces. Washington : American Chemical Society. 2012, vol 4, no 5, p. 2643-2651.

A. Marozienė, A. Nemeikaitė-Čėnienė, R. Vidžiūnaitė, N. Čėnas. Correlation between mammalian cell cytotoxicity of flavonoidsand the redox potential of phenoxyl radical/phenol couple // Acta Biochimica Polonica. Warszawa : Polskie Towarzystwo Biochemiczne. 2012, Vol. 59, no. 2, p. 299-305.

A. Misiūnas, G. Niaura, J. Barauskas, R. Meškys, R. Rutkienė, V. Razumas, T. Nylander. Horse heart cytochrome c entrapped into the hydrated liquid-crystalline phases of phytantriol: X-ray diffraction and Raman spectroscopic characterization // Journal of colloid and interface science. Maryland Heights : Academic Press. 2012, Vol. 378, iss. 1, p. 232-240.

R. Baronas, J. Kulys, K. Petrauskas, J. Razumienė. Modelling Carbon Nanotubes-Based Mediatorless Biosensor // Sensors. Basel : MDPI AG. 2012, Vol. 12, iss. 7, p. 9146-9160.

D. Šimelevičius, R. Baronas, J. Kulys. Modelling of Amperometric Biosensor Used for Synergistic Substrates Determination // Sensors. Basel : MDPI AG. 2012, Vol. 12, iss. 4, p. 4897-4917.

V. Ašeris, R. Baronas, J. Kulys. Modelling the biosensor utilising parallel substrates conversion // Journal of Electroanalytical Chemistry. Lausanne : Elsevier S.A. 2012, Vol. 685, p. 63-71.

F. Tiberg, M. Johnsson, M. Jankunec, J. Barauskas. Phase behavior, functions, and medical applications of soy phosphatidylcholine and diglyceride lipid compositions // Chemistry letters. Tokyo : Chemical Society of Japan. 2012, vol. 41, no 10, p. 1090-1092.

R. Baronas, Ž. Ledas, R. Šimkus. Modeling and Simulation of Bacterial Self-Organization in Circular Container Along Contact Line as Detected by Bioluminescence Imaging // International Journal On Advances in Systems and Measurements. Ithaca : IARIA. 2012, Vol. 5, nr. 3-4, p. 154-163.

Materials of conferences

V. Laurinavičius, J. Razumienė, B. Kurtinaitienė, J. Stankevičiūtė, R. Meškys. Reagentless and mediator-based electrochemical biosensors for food industry and medicine // IEEE sensors 2012. Taipei, 2012, p. 1549-1552.

R. Baronas, Ž. Ledas, R. Šimkus. Computational modeling of self-organization in a liquid phase bacterial bioluminescent biosensor // ECCOMAS 2012 : 6th European congress on computational methods in applied sciences and engineering : CD-ROM proceedings, Vienna, September 10-14, 2012. Vienna, 2012, p. [1-8].

V. Ašeris, R. Baronas, J. Kulys. Effects of diffusion limitations on the response of biosensors utilizing parallel substrates conversion // ECCOMAS 2012 : 6th European congress on computational methods in applied sciences and engineering : CD-ROM proceedings, Vienna, September 10-14, 2012. Vienna, 2012, p. [1-10].

COOPERATION

Malmö University (Sweden)
Lund University (Sweden)
Camurus (Sweden)
Gaziantep University (Turkey)
Lithuanian Centre of Agriculture and Forestry (Lithuania)
Lithuanian Institute of Agriculture (Lithuania)
Faculty of Mathematics and Informatics, Vilnius University (Lithuania)
Faculty of Chemistry, Vilnius University (Lithuania)
Faculty of Natural Sciences, Vilnius University (Lithuania)

OTHER SCIENTIFIC ACTIVITIES

Prof. V. Laurinavičius –

member of the Lithuanian Academy of Sciences;
member of the Lithuanian Biotechnology Association;
member of the Vilnius University Senate;
Lithuania member-expert of EC IDEAS Programme Committee.

Prof. J. Kulys –

member of the Lithuanian Academy of Sciences;
member of the Research Council of Lithuania;
member of the European Biotechnology Association;
member of the Lithuanian Biotechnology Association;
member of the Lithuanian Biochemistry Association;
National Scientific Awards (1987, 2002);
Award of Baltic Assembly (1995);
editor of the journal Biosensors & Bioelectronics;
editorial board member of the journals:

                            The Open Nanoscience Journal,
                            The Open Enzyme Inhibition Journal,
                            The Open Biotechnology Journal,
                            Biologija (Biology),
                           Nonlinear analysis. Modelling and Control.

Dr. J. Razumienė –

member of International Society of Electrochemistry (ISE), http://www.ise-online.org;
member of Management Committee of COST Action CM0701, http://www.cost-cascat.polimi.it;
member of Lithuanian Biochemical Society (LBD).

Dr. R. Vidžiūnaitė –

expert of the Research Council of Lithuania;
docent (part time) at the Department of Chemistry and Bioengineering of Vilnius Gediminas Technical University;
member of the Lithuanian Biochemical Society (LBD).

DEPARTMENT OF BIOELECTROCHEMISTRY AND BIOSPECTROSCOPY

12 Mokslininkų, LT-08662 Vilnius
Tel. 272 9144, fax 272 9196
E-mail:
www http://www.bchi.vu.lt

Head – Dr. Gintaras Valinčius

STAFF

Chief research fellows: Dr. G. Valinčius, Habil.dr. G. Niaura.
Junior research fellows: Dr. A. Bulavas, Dr. R. Budvytytė.
Senior specialist: B. Rakovska.
Doctoral students: T. Ragaliauskas, M. Talaikis, N. Dzingelevičius, J. Latynis, M. Mickevičius.

RESEARCH INTERESTS

Spectroelectrochemistry of proteins and biologically relevant redox species
Self-organizing lipid systems, including phospholipid bilayer membranes
Interaction with proteins
Development of measurement techniques: electrochemical impedance spectroscopy, modulated surface stress, sum-frequency generation, and surface enhanced Raman spectroscopy

RESEARCH PROJECTS CARRIED OUT IN 2012

Projects Supported by University Budget

Development and Optimization of Model Self-Assembling Systems for Research of Biological Process and Biotechnology. Doc. Dr. G. Valinčius. 2011–2015.

The electrochemical impedance spectra (EIS) of tethered bilayer membranes (tBLMs) were analyzed, and the analytical solution for the spectral response of membranes containing natural or artificially introduced defects was derived. The analysis carried out shows that the EIS features of an individual membrane defect cannot be modelled by conventional electrical elements. The primary reason for this is a complex nature of an impedance of the submembrane ionic reservoir separating the phospholipid layer and the solid support. We demonstrate that its EIS response, in case of radially symmetric defects, is described by the Hankel functions of a complex variable. Therefore, neither the impedance of the submembrane reservoir, nor the total impedance of tBLMs can be modelled using the conventional elements of the equivalent electrical circuits of interfaces. The presented analysis provides practical tools to evaluate the defect density in tBLMs, which could be utilized in tBLM-based biosensor applications. Alternatively, if the parameters of the defects, e.g., ion channels, such as the diameter and the conductance are known, the EIS data analysis provides possibility to estimate other physical parameters of the system, such as thickness of the submembrane reservoir, and its conductance. Finally, current analysis demonstrates possibility to discriminate between the situations, in which the membrane defects are evenly distributed or clustered on the surface of tBLMs. Such sensitivity of EIS could be used for elucidation of the mechanisms of interaction between the proteins and membranes. Surface-enhanced Raman spectroscopy (SERS) was used to detect different spectral features of small (1-2 nm) and large (5-10 nm) synthetic amyloid Ab-42 oligomers, exhibiting high and no detectable neurotoxicity, respectively. Adsorption of peptides at self-assembled monolayers (SAM) terminated by methyl and pyridinium groups was employed to differentiate toxic and non-toxic oligomers. Three SAMs were analyzed: hydrophobic heptanethiol (HT) and octadecanethiol (ODT) as well as positively charged N-(6-mercapto)hexylpyridinium (MHP) chloride. SERS study revealed two-fold adsorption effect, changes in the monolayer structure and appearance of new bands associated with the adsorbed peptides. A band at 1387 cm-1, observed as a result of the SAM and Ab-42 interaction, is tentatively assigned to the peptide symmetric stretching vibration of carboxylate groups, and appears to be the most prominent spectral feature distinguishing toxic oligomers from the non-toxic Ab-42 forms. This band was identified in the spectra of Ab-42 adsorption on heptanethiol and MHP monolayers, while no clear perturbations were observed in the case of ODT monolayer.

Projects, Programmes, Issues Supported by the Research Council of Lithuania

Project Surface Tethered Bilayer Phospholipid Membranes for Functional Reconstitution of Proteins – Dr. G. Valinčius. 2011–2012.

Project Separation and Characterization of Toxic Amyloid Peptide Species Implicated in Alzheimer’s Disease – Dr. G. Valinčius. 2011–2012.

Project: Molecular Mechanisms of Alzheimer’s Pathogenesis. Dr. G. Valinčius. 2012-2014.

MAIN PUBLICATIONS

Articles

G. Valinčius, T. Meškauskas, F. Ivanauskas. Electrochemical Impedance Spectroscopy of Tethered Bilayer Membranes // Langmuir. Washington : American Chemical Society. 2012, vol. 28, iss. 1, p. 977-990.

I. Tijūnėlytė, J. Babinot, M. Guerrouache, G. Valinčius. Hydrophilic monolith with ethylene glycol-based grafts prepared via surface confined thiol-ene click photoaddition // Polymer. Oxford: Elsevier SCI LTD. 2012, vol. 53, no. 1, p. 29-36.

V. Voiciuk, G. Valinčius, R. Budvytytė, A. Matijoška, I. Matulaitienė, G. Niaura. Surface-enhanced Raman spectroscopy for detection of toxic amyloid Ī² oligomers adsorbed on self-assembled monolayers // Spectrochimica Acta. Part A: Molecular and Biomolecular Spectroscopy. Amsterdam : Elsevier BV. 2012, Vol. 95, p. 526-532.

COOPERATION

Institute of Biomedical Research of LSMU, Kaunas (Lithuania)
EKSPLA, Vilnius (Lithuania)
Univ Paris Est, Inst Chim & Mat Paris Est (France)
Ohio State University, Columbus, OH (USA)
Carnegie Melon University, Pittsburgh, PA (USA)
National Institute of Standards and Technology, Gaithersburg, MD (USA)
University of Maryland Institute of Biotechnology, Rockville, MD (USA)
University of Cracow, Cracow (Poland)

OTHER SCIENTIFIC ACTIVITIES

Dr. G. Valinčius –

Lithuanian representative in European Strategy Forum for Research Infrastructures (ESFRI);
Lithuanian representative in the Program Committee of Infrastructures of the specific program Capacities of FP7;
member of the Valley Supervisory Commission at the Ministry of Education and Science of Lithuania;
member of the American Chemical Society.

Habil. Dr. G. Niaura –

member of the Lithuanian Academy of Sciences.

Dr. R. Budvytytė –

member of the American Biophysical Society.

DEPARTMENT OF BIOORGANIC COMPOUNDS CHEMISTRY

12 Mokslininkų, LT-08662 Vilnius
Tel. 272 9144, fax 272 9196
E-mail:
www http://www.bchi.vu.lt

Head – Dr. Regina Jančienė

STAFF

Senior research fellows: Dr. A. Palaima, Dr. R. Jančienė.
Research fellows: Dr. A. Klimavičius, Dr. Z. Staniulytė, Dr. L. Kosychova.
Junior research fellow: Dr. R. Sirutkaitis.

RESEARCH INTERESTS

Synthesis of heterocyclic, amino acid and polyether derivatives

RESEARCH PROJECTS CARRIED OUT IN 2012

Projects Supported by University Budget

Investigation of the Synthesis Methods for Stereoisomeric Cis/Trans-1,4-Cyclohexanedicarboxylic Acid Derivatives and Annelated Heterocycles Having 1,5-Benzodiazepine and Quinazoline Fragments. Dr. A. Palaima, Dr. R. Jančienė. 2011–2013.

Interaction of 2,5-cyclohexadien-1,4-dione (p-benzoquinone) with o-, m-, p-substituted aniline derivatives was investigated. p-Benzoquinone reacted with aqueous solutions of various amines rather easily, and 2-arylamino-1,4-benzoquinones were formed. It was established that the yield and purity of 2-substituted benzoquinones depended on the reaction time, temperature, dilution and the ratio of reactants. The course of reactions was greatly influenced by the nature of substituents in the aromatic ring of amines. The synthesized 2-arylamino-cyclohexa-2,5-diene-1,4-diones were tested as new materials for electrochemical biocatalysis.
The synthesis of dodecaethylene glycol from dimezyltetraethylene glycol and an excess of tetraethylene glycol was studied. It was established that the impurities of this reaction were difficult to separate from the target product and their amount greatly depended on the reaction temperature, time, dilution, and stirring intensity.
Continuing our search for methods of synthesis of fused 1,5-benzodiazepine derivatives, we studied the transition metal induced reductive N-heterocyclization of 5-acyl-1-(2-nitrobenzoyl)-1,3,4,5-tetrahydro-2H-1,5-benzodiazepin-2-ones and synthesized derivatives carrying 6,7-dihydroquinazolino[3,2-a][1,5]benzodiazepine fragment. Quantum-chemical reactivity descriptors were calculated to explain the peculiarities of reductive cyclization.

Ordered Research Projects

Project: Investigation of Regeneration Conditions of Butylacetate and Negative Developer and Manufacturing of their Experimental Batches. Contract with UAB Vilniaus Ventos puslaidininkiai. Dr. A. Klimavičius. 2012.

Project: Preparation of Nickel Brightener. Contract with UAB Elonika. Dr. A. Palaima. 2012.

Project: Preparation of Emulsifier-solubilizer. Contract with UAB Ekochemeta. Dr. A. Palaima. 2012.

Project: Synthesis of Pantetine-4‘,4‘‘-diphosphate. Contract with UAB Fermentas. Dr. A. Palaima. 2012.

Project: Development of Multi-Step Methods for Synthesis of Organic Products and Manufacturing of their Experimental Batches. Contract with Ramidus AB. Dr. A. Palaima. 2008–till termination.

MAIN PUBLICATIONS

Articles

R. Butkutė, A. Sinycina, L. Kosychova. Alternatyvaus kuro panaudojimo degaluose tyrimas // Technologijos mokslo darbai Vakarų Lietuvoje / Klaipėdos universitetas. Klaipėda : Klaipėdos universiteto leidykla, 2010. 2012, T. 8, p. 280-284.

A. Voitechovičius, K. Karpavičius, A. Voitechovičiūtė, S. Tumkevičius, P. Adomėnas. Synthesis of novel 4,6-diaryl- and 4,6-bis(2-arylvinyl)-pyrimidines // Latvian Journal of Chemistry. Salaspils : Institute of Inorganic Chemistry of the Riga Technical University. 2012, no. 1 : Materials of the Paul Walden 7th Symposium on organic chemistry (Riga, September 12-13, 2011), p. 77.

Materials of conferences

J. Šarlauskas, Ž. Anusevičius, V. Miliukienė, V. Petrikaitė, L. Misevičienė, A. Marozienė, K. Krikštopaitis, N. Čėnas, A. Pavilonis, A. Klimavičius, J. Meškauskas. Heterocyclic N-oxides - benzofuroxans and quinoxaline N,N'-dioxides - synthesis, properties and biological activity studies // Organinė chemija = Organic chemistry : mokslinės konferencijos pranešimų medžiaga. Kaunas : Technologija, 2012, p.76-78.

R. Urbonas, S. Tumkevičius. 2,4,7-Triaril-7H-pirolo[2,3-dpirimidinų sintezė, panaudojant Pd(0), Ni(0) ir Cu(I) katalizuojamas reakcijas // Chemija ir cheminė technologija : studentų mokslinės konferencijos pranešimų medžiaga. Klaipėda, 2012, gegužės 15 d. Klaipėda : Klaipėdos universiteto leidykla, 2012, p. 214-216.

COOPERATION

CC “Fermentas” (Lithuania)
CC “Vilniaus Ventos puslaidininkiai” (Lithuania)
CC “Ekochemeta” (Lithuania)
CC innovative firm “MKDS” (Lithuania)
Ramidus AB (Sweden)
Polypure (Norway)
Synthon Chemicals GmbH & Co.KG (Germany)

DEPARTMENT OF XENOBIOTICS BIOCHEMISTRY

12 Mokslininkų, LT-08662 Vilnius
Tel. 272 9144, fax 272 9196
E-mail:
www http://www.bchi.vu.lt

Head – Habil. Dr. Narimantas Čėnas

STAFF

Chieff research fellow: Habil. Dr. N. Čėnas.
Senior research fellows: Dr. Ž. Anusevičius, Dr. J. Šarlauskas.
Research fellows: Dr. A. Marozienė, Dr. V. Miliukienė, Dr. L. Misevičienė, Dr. H. Nivinskas.
Doctoral student: R. Jarašienė.

RESEARCH INTERESTS

Flavoenzyme catalysis
Bioreductive activation/detoxification of redox active xenobiotics
Cytotoxicity and therapeutic (anticancer, antiparasitic) action of quinones, nitroaromatic compounds, and polyphenols
Cytotoxicity and biodegradation of nitroaromatic environmental pollutants

RESEARCH PROJECTS CARRIED OUT IN 2012

Projects Supported by University Budget

The Studies of Physicochemical, Enzymatic, and Cytotoxic Properties of 6,7-Dinitroquinoxaline -2,3-Dione (DNQX) and its Analogues. Habil. Dr. N. Čėnas. 2011–2016.

We have examined the voltammetric characteristics of neuromodulating agent 6,7-dinitroquinoxa-line-2,3-dione (DNQX), its 6-mononitro derivative, and quinoxaline-2,3-dione, their reactivity towards single-electron transferring flavoenzymes NADPH:cytochrome P-450 reductase and NADPH:adrenodoxin reductase/adrenodoxin complex, their reactivity towards a two-electron transferring flavoenzyme DT-diaphorase, and their cytotoxicity in bovine leukaemia virus-tarnsformed lamb kidney fibroblasts (line FLK). The cytotoxicity of the above nitroheterocyclic compounds was prooxidant, i.e., having been prevented by the antioxidants, and that it correlated with the rates of their single-electron enzymatic reduction and voltammetric reduction potentials, which were similar to those of o-dinitrobenzene and p-nitroacetophenone, respectively. The reactions catalyzed by DT-diaphoresis exerted negligible role in the cytotoxicity of compounds. The cytotoxicity of quinoxaline-2,3-dione was much lower, this compound was inactive towards above mentioned flavoenzymes. Thus, our data characterize the mode of cytotoxicity of DNQX, which may be important as a side-effect of this drug.

Projects, Programmes, Issues Supported by Research Council of Lithuania

Heterocyclic N-Oxides: Synthesis, Cytotoxicity, and Interaction with Target Enzymes, Dr. J. Šarlauskas. 2011–2012.

Molecular Mechanisms of Toxicity and Antitumor Activity of Quinones and Polyphenols: Enzymatic Redox Cytotoxicity, Signal Transduction, and Proteomics. Habil. dr. N. Čėnas, 2011–2015.

International Science Programmes and Projects

COST Action CM0801: New Drugs for Neglected Diseases. Dr. J. Šarlauskas, Habil. Dr. N. Čėnas. 2009–2012.

MAIN PUBLICATIONS

Articles

G. Bikulčius, A. Ručinskienė, V. Bukelskienė, A. Selskis, A. Grigucevičienė. Formation of Conversion Coatings on Magnesium Alloy AZ31 in Solutions Containing Nicotinic Acid // Materials science = Medžiagotyra / Kaunas University of Technology, Academy of Sciences of Lithuania. Kaunas : Technologija. 2012, vol. 18, no. 2, p. 123-127.

V. Miliukienė, J. Šarlauskas, K. Krikštopaitis, A. Šaikūnas, N. Čėnas. Possible modes of cytotoxicity of high energy aliphatic nitroesters and N-nitramines for mouse splenocytes // Chemija / Lietuvos mokslų akademija. Vilnius : Lietuvos mokslų akademijos leidykla. 2012, t. 23, nr. 2, p. 141-146.

V. Intaitė, A. Voskienė, R. Vaickelionienė, G. Mikulskienė, V. Mickevičius. Synthesis and structure of new 1,3-disubstituted 5-oxopyrrolidine derivatives // Chemija / Lietuvos mokslų akademija. Vilnius : Lietuvos mokslų akademijos leidykla. 2012, t. 23, nr. 1, p. 52-60.
J. Šarlauskas, Ž. Anusevičius, A. Misiūnas. Benzofuroxan (Benzo[1,2-c]1,2,5-oxadiazole N-oxide) derivatives as potential energetic materials: studies on their synthesis and properties // Central European Journal of Energetic Materials. Warsaw : Instytut Przemyslu Organicznego. 2012, Vol. 9, no. 4, p. 365-386.

M. Malinauskas, D. Baltriukienė, A. Kraniauskas, P. Danilevičius, R. Jarašienė, R. Širmenis, A. Žukauskas, E. Balčiūnas, V. Purlys, R. Gadonas, V. Bukelskienė, V. Sirvydis, A. Piskarskas. In vitro and in vivo biocompatibility study on laser 3D microstructurable polymers // Applied physics. A. Materials science & processing. Heidelberg : Springer. 2012, Vol. 108, no. 3, p. 751-759.

P. Danilevičius, S. Rekštytė, E. Balčiūnas, A. Kraniauskas, R. Jarašienė, R. Širmenis, D. Baltriukienė, V. Bukelskienė, R. Gadonas, M. Malinauskas. Micro-structured polymer scaffolds fabricated by direct laser writing for tissue engineering // Journal of biomedical optics. Bellingham : SPIE. 2012, Vol. 17, no. 8, art. no. 081405 (1-7).

Ž. Anusevičius, L. Misevičienė, J. Šarlauskas, N. Rouhier, J. Jacquot, N. Čėnas. Quinone- and nitroreductase reactions of Thermotoga maritima peroxiredoxin-nitroreductase hybrid enzyme // Archives of Biochemistry and Biophysics. Maryland Heights : Academic Press. 2012, Vol. 528, no. 1, p. 50-56.

L. Labanauskas, V. Dudutienė, G. Urbelis, J. Šarlauskas, J. Sūdžius, D. Matulis, R. Striela, A. Žilinskas. Synthesis of substituted 2Ī»4Ī´2-[1,2,3]thiadiazolo[3,4-c]benzimidazoles and 2Ī»4Ī´2-[1,2,3,5]thiatriazolo[3,4-c]benzimidazoles // ARKIVOC : Archive for Organic Chemistry. Gainesville : ARKAT USA, Inc. 2012, part. 8, p. 17-26.

Materials of conferences

J. Šarlauskas. Some derivatives of benzofuroxan (benzo[1,2-c]1,2,5- oxadiazole N-oxide): recent studies on the synthesis and properties // New trends in research of energetic materials : proceedings of 15th international seminar, Czech Republic, April 18-20, 2012. Pardubice : University of Pardubice, 2012, p. 780-789.
J. Šarlauskas, Ž. Anusevičius, V. Miliukienė, V. Petrikaitė, L. Misevičienė, A. Marozienė, K. Krikštopaitis, N. Čėnas, A. Pavilonis, A. Klimavičius, J. Meškauskas. Heterocyclic N-oxides - benzofuroxans and quinoxaline N,N'-dioxides - synthesis, properties and biological activity studies // Organinė chemija = Organic chemistry : mokslinės konferencijos pranešimų medžiaga. Kaunas : Technologija, 2012, p.76-78.

COOPERATION

Universita degli Studi di Milano (Italy)
Museum National d‘Histoire Naturelle, Paris (France)
Biochemie-Zentrum der Universität Heidelberg (Germany)
University of Manchester (United Kingdom).

OTHER SCIENTIFIC ACTIVITIES

Habil. Dr. N. Čėnas –

expert of the Research Council of Lithuania;
expert of the 7 FP project evaluation, http://cordis.europa.eu/fp7/experts_en.html.

Dr. J. Šarlauskas –

expert of the 7 FP project evaluation, http://cordis.europa.eu/fp7/experts_en.html.

DEPARTMENT OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY

12 Mokslininkų, LT-08662 Vilnius
Tel. 272 9144, fax 272 9196
E-mail:
www http://www.bchi.vu.lt

Head – Dr. Rolandas Meškys

STAFF

Chief research fellow: Dr. R. Meškys.
Senior research fellows: Dr. L. Truncaitė, Dr. I. Bachmatova, Dr. J. Urbonavičius.
Research fellows: Dr. A. Zajančkauskaitė, Dr. L. Kalinienė; Dr. L. Marcinkevičienė, Dr. R. Rutkienė,
Dr. V. Časaitė, Dr. D. Tauraitė.
Junior research fellows: Dr. R. Gasparavičiūtė, R. Meškienė, Dr. S. Povilionienė, Dr. R. Stanislauskienė.
Senior assistants: Dr. J. Kiuberis, S. Kutanovas.
Doctoral students: Ž. Strazdaitė-Žielienė, E. Šimoliūnas, J. Stankevičiūtė, J. Vaitekūnas, L. Adamonytė.

RESEARCH INTERESTS

Molecular biology and genetics of bacteria and bacteriophages

RESEARCH PROJECTS CARRIED OUT IN 2012

Projects Supported by University Budget

Investigation of the Escherichia Coli Endoribonuclease G Activity Changes during Infection of Bacteriophage T4. Dr. L. Truncaitė. 2011–2015.

A sequence specific RegB endoribonuclease encoded by T4 related bacteriophages is usually toxic to the Escherichia coli cells when cloned and expressed in high quantities. In this study we cloned into expression vectors regB genes from different T4 like bacteriophages, which infect either E. coli (phages RB69, RB49, vB_EcoM_VR7) or Aeromonas salmonicida (phage 44RR). Recombinant endoribonucleases were tested for their activities in vivo by analysing their own mRNAs by primer extension analysis. All RegB proteins were active and showed high toxicity to the E. coli cells after induction. However, after mutagenesis of cloned regB genes we were able to express RegB proteins, which were less toxic to the cells.

Screening and Identification of Coumarins-Degrading Microorganisms. Application of NAD- And PQQ-Dependent Alcohol Dehydrogenases for Bioconversion. Dr. R. Meškys. 2011–2014.

Rhodococcus sp. K5 capable of growth on coumarin and melilotic acid was isolated for the first time. The coumarin inducible proteins were identified by SDS-PAGE and de novo protein sequencing. Quinohemoprotein alcohol dehydrogenases (ADHs) are attractive catalysts because of their wide substrate specificity and non-diffusible cofactor. ADH IIG from Pseudomonas putida HK5 was capable to oxidize various primary or secondary aliphatic and cyclic amino alcohols stereo selectively. An optimal pH range for ADH IIG activity was shifted to more acidic side (pH 4–6) when amino alcohols were used as substrates. The ADH IIG covalently immobilized on silica gel by cross-linking with glutaraldehyde or 1-ethyl-3(3-dimethylaminopropyl) carbodiimide exhibited a higher storage stability and thermo stability as well as an improved activity in the presence of organic solvents comparing with the free enzyme. The affinity of ADH IIG to substrates was not significantly changed in the presence of organic solvents or after the immobilization of the enzyme.

Projects, Programmes, Issues Supported by Research Council of Lithuania

Project: Pyridines and Pyrazines: Biodegradation and Biocatalytic Synthesis. Dr. R. Meškys. 2011–2012.

Project: Development of Methods for Screening and Expression of Baeyer-Villiger Monooxygenases. Dr. R. Meškys. 2012–2014.

Project: tRNA Modification Pathways – Evidence of the Enzyme Evolution. Dr. J. Urbonavičius. 2012–2014.

Project: Adaptation of Invasive Species and their Impact on Aquatic Ecosystems of Different Complexity. Dr. R. Meškys. 2012–2015.

Projects, Programmes, Issues Supported by Agency for Science, Innovation and Technology (MITA), Lithuania

Project: Search of New and Genetically Modified Oxidoreductases for Creation of Biofuel Cells. Dr. R. Meškys. 2012–2013.

MAIN PUBLICATIONS

L. Karvelis, R. Gasparavičiūtė, R. Meškys. Characterization of 2,5-dihydroxypyridine dioxygenases from Sinorhizobium sp. L1 // Biologija. Vilnius : Lietuvos mokslų akademijos leidykla. 2012 vol. 58, nr. 2, p. 43-50.

L. Truncaitė, E. Šimoliūnas, A. Zajančkauskaitė, L. Kalinienė, R. Mankevičiūtė, J. Staniulis, V. Klausa, R. Meškys. Bacteriophage vB_EcoM_FV3: a new member of "rV5-like viruses" // Archives of Virology. Wien : Springer. 2012, Vol. 157, no 12, p. 2431-2435.

R. Rimavičiūtė, I. Dumalakienė, R. Vilienė, R. Meškienė, R. Meškys. Construction of a DNA vector system for Mycoplasma gallinarum // Romanian biotechnological letters. Bucharest : University of Bucharest. 2012, Vol. 17, no. 4, p. 7533-7539.

R. Stanislauskienė, R. Gasparavičiūtė, J. Vaitekūnas, R. Meškienė, R. Rutkienė, V. Časaitė, R. Meškys. Construction of Escherichia coli-Arthrobacter-Rhodococcus shuttle vectors based on a cryptic plasmid from Arthrobacter rhombi and investigation of their application for functional screening // FEMS Microbiology Letters. Oxford : Wiley-Blackwell Publishing Ltd. 2012, Vol. 327, iss. 1, p. 78-86.

E. Šimoliūnas, L. Kalinienė, L. Truncaitė, V. Klausa, A. Zajančkauskaitė, R. Meškys. Genome of Klebsiella sp.-infecting bacteriophage vB_KleM_RaK2 // Journal of Virology. Washington : American Society for Microbiology. 2012, Vol. 86, no. 9, p. 5406.

V. Bukauskas, A. Šetkus, I. Šimkienė, S. Tumėnas, I. Kašalynas, A. Rėza, G. Babonas, V. Časaitė, S. Povilonienė, R. Meškys. Solid surface dependent layering of self-arranged structures with fibril-like assemblies of alpha-synuclein // Applied surface science. Amsterdam : Elsevier Science Bv. 2012, Vol. 258, iss. 10, p. 4383-4390.

Materials of conferences

COOPERATION

Centre for Physical Sciences and Technology (Lithuania)
Nature Research Centre (Lithuania)

OTHER SCIENTIFIC ACTIVITIES

Dr. R. Meškys –

expert of the 7 FP projects, http://cordis.europa.eu/fp7/experts_en.html.

PROTEOMICS CENTRE

12 Mokslininkų, LT-08662 Vilnius
Tel. 272 9144, fax 272 9196
E-mail:
www http://www.bchi.vu.lt

Head – Dr. MindaugasValius

STAFF

Research fellow: Dr. A. Kaupinis.
Junior research fellow: Dr. M. Ger.
Doctoral students: Z. Žitkus, D. Kučiauskas, A. Androšiūnaitė.

RESEARCH INTERESTS

Proteomics analysis of cell signalling pathways
Elucidation of nanoparticle and artificial microstructures on cell proliferation and differentiation Studies of Ras-dependent signalling pathways in normal and cancer cells

RESEARCH PROJECTS CARRIED OUT IN 2012

Projects Supported by University Budget

Research of Molecular Mechanisms Regulating Eukaryotic Cells Proliferation, Differentiation and Apoptosis. (Joint project with Department of Developmental Biology). Prof. R. Navakauskienė. 2011–2015.

To Elucidate of RasGAP and Nck1 Interaction Mechanisms. Dr. M. Valius.

Previously we have shown that Ras GTPase activating protein (RasGAP) constantly interacts with adaptor protein Nck1; this interaction increases RasGAP ability to inactivate Ras. Now we show that this interaction can take place only in the cells that have contact with the substrate. Detachment of cells from the substrate leads to dissipation of RasGAP-Nck complex, down-regulation of RasGAP activity and increase of active Ras species in the suspended cells. The data provide mechanistic explanation how the attachment of adherent cells to the substrate increases their Ras activity, which serves as anti-apoptotic factor and increases attached cell survival.

Projects, Programmes, Issues Supported by Research Council of Lithuania

Project: Creation of Biologically Active Regular Three-Dimensional Structures for Tissue Molecular Bioengineering. Dr. M. Valius. 2011–2013.

International Science Programmes and Projects

COST Action BM0702: Urine and Kidney Proteomics (EuroKUP). Dr. M. Ger. 2010–2012.

MAIN PUBLICATIONS

A. Jasilionis, A. Kaupinis, M. Ger, M. Valius, D. Čitavičius, N. Kuisienė. Gene expression and activity analysis of the first thermophilic U32 peptidase // Central European Journal of Biology. Warsaw : Versita. 2012, Vol. 7, no. 4, p. 587-595.

COOPERATION

Schepens Eye Research Institute, Harvard Medical School (USA)

DEPARTMENT OF MOLECULAR CELL BIOLOGY

12 Mokslininkų, LT-08662 Vilnius
Tel. 272 9144, fax 272 9196
E-mail:
www http://www.bchi.vu.lt

Head – Prof. Dr. Rūta Navakauskienė

STAFF

Senior research fellow: Dr. A. Kalvelytė.
Research fellows: Dr. G. Treigytė, Dr. J. Savickienė, Dr. V. V. Borutinskaitė, Dr. A. Imbrasaitė.
Junior research fellows: A. Stulpinas, N. Krestnikova.
Doctoral students: S. Baronaitė, G. Vištartaitė.

RESEARCH INTERESTS

Studies of molecular mechanisms in normal and cancer cells to determine new possibilities of leukaemia differentiation therapy
Identification of proteins that might be involved in signalling events that mediate cancer cell differentiation

RESEARCH PROJECTS CARRIED OUT IN 2012

Projects Supported by University Budget

Research of Molecular Mechanisms Regulating Eukaryotic Cells Proliferation, Differentiation and Apoptosis. Prof. R. Navakauskienė. 2011–2015.

The aim was to study the effect of histone deacetylase inhibitor EGCG on differentiation and apoptosis of human cancer cells.
Epigenetic changes such as histone acetylation and DNA methylation play an important role in human cancer cells. Chemical agents that affect histone deacetylases and DNA methyltransferases could be potential targets in cancer treatment. In this work it was found out that natural DNA methyltransferases inhibitor EGCG reduces cell proliferation and increases cell death – apoptosis, in both NB4 leukaemia and HeLa cervical cancer cells. We have shown by flow cytometry method that EGCG and its combination with retinoic acid induce differentiation in NB4 cells.

Projects, Programmes, Issues Supported by the Research Council of Lithuania

Project: Multiplexed Molecular Biomarker study for Leukaemia. Prof. Dr. R. Navakauskienė. 2012–2015.

Participation in the project Bee Products Enriched with Plant Components, the Composition and Properties. 2012–2015.

International Science Programmes and Projects

COST Action BM0801: Translating Genomic and Epigenetic Studies of MDS and AML (EuGESMA). Prof. R. Navakauskienė. 2011–2012.

COST Action CM1106: Chemical Approaches to Targeting Drug Resistance in Cancer Stem Cells (StemChem). Prof. R. Navakauskienė. 2011–2015.

Projects supported by the European Social Fund

Participation in the project supported by the European Social Fund under National Integrated Programme Biotechnology & Biopharmacy, grant VP1-3.1-SMM- 08-K01-005. 2012–2015.

MAIN PUBLICATIONS

Articles

R. Navakauskienė, G. Treigytė, V. Borutinskaitė, D. Matuzevičius, D. Navakauskas, K. Magnusson. Alpha-dystrobrevin and its associated proteins in human promyelocytic leukemia cells induced to apoptosis // Journal of proteomics. Amsterdam : Elsevier BV. 2012, vol. 75, no 11, p. 3291-3303.

J. Savickienė, G. Treigytė, V. Borutinskaitė, R. Navakauskienė. Antileukemic activity of combined epigenetic agents, DNMT inhibitors zebularine and RG108 with HDAC inhibitors, against promyelocytic leukemia HL-60 cells // Cellular and molecular biology letters. Wroclaw : Uniwersytet Wroclawski. 2012, vol. 17, no 4, p. 501-525.

A. Stulpinas, A. Imbrasaitė, A. Kalvelytė. Daunorubicin induces cell death via activation of apoptotic signalling pathway and inactivation of survival pathway in muscle-derived stem cells // Cell Biology and Toxicology. Dordrecht : Springer Netherlands. 2012, vol. 28, no. 2, p. 103-114.

J. Savickienė, G. Treigytė, A. Jazdauskaitė, V. Borutinskaitė, R. Navakauskienė. DNA methyltransferase inhibitor RG108 and histone deacetylase inhibitors cooperate to enhance NB4 cell differentiation and E-cadherin re-expression by chromatin remodelling // Cell Biology International. London : Portland Press Ltd. 2012, Vol. 36, iss. 11, p. 1067-1078.

J. Savickienė, G. Treigytė, V. Jonušienė, R. Bružaitė, V. Borutinskaitė, R. Navakauskienė. Epigenetic changes by zebularine leading to enhanced differentiation of human promyelocytic leukemia NB4 and KG1 cells // Molecular and Cellular Biochemistry. New York : Springer New York LLC. 2012, Vol. 359, iss. 1-2, p. 245-261.

V. Borutinskaitė, K. Magnusson, R. Navakauskienė. Histone deacetylase inhibitor BML-210 induces growth inhibition and apoptosis and regulates HDAC and DAPC complex expression levels in cervical cancer cells // Molecular biology reports. Dordrecht : Springer. 2012, vol. 38, no 5, p. 10179-10186.

COOPERATION

Linkoping University (Sweden)

DEPARTMENT OF BIOLOGICAL MODELS (former VIVARIUM)

12 Mokslininkų, LT-08662 Vilnius
Tel. 272 9144, fax 272 9196
E-mail:
www http://www.bchi.vu.lt

Head –Dr. Virginija Bukelskienė

STAFF

Senior Research fellow: Dr. V. Bukelskienė.
Research fellow: Dr. D. Baltriukienė.

RESEARCH INTERESTS
Adult stem cell research, cytotherapy, cell technologies

RESEARCH PROJECTS CARRIED OUT IN 2012

Projects Supported by University Budget

Research of Molecular Mechanisms Regulating Eukaryotic Cells Proliferation, Differentiation and Apoptosis. (Joint project with the Department of Developmental Biology). Prof. R. Navakauskienė. 2011–2015.

JNK Signalling after the Treatment with Natural Toxins in the Kidney-Derived Cells. Dr. V. Bukelskienė.

Natural toxins referred to as mycotoxins producing mould species are extremely common. They can grow on a wide range of substrates under environmental conditions, and are linked to a variety of acute and chronic adverse health effects. In our study, effect of mycotoxins deoxynivalenol (DON) and ochratoxin A (OTA) were studied in model kidney-derived cells (BHK21). The results were compared with processes in model liver cell line (MH-22A). We have found that sensitivity to OTA and DON of both kinds of cells was similar and rather small in comparison to other kinds of cells. After 24 h treatment with tested mycotoxins, we observed the activation of c-Jun N-terminal kinase (JNK) in MH-22A cell culture, but this effect was inappreciable in BHK21 cell culture. However, we have registered the antiapoptotic role of JNK in both kidney- and liver-derived cells. Investigating the role of c-Jun, which is the main substrate of JNK, we have found that OTA and DON induced c-jun expression which correlated with proapoptotic effect of c-Jun.

Projects, Programmes, Issues Supported by the Research Council of Lithuania

Project: Evaluation of Gingival Fibroblast Adhesion Properties and their Usage in Regeneration of Soft Tissue. Project participants: Dr. V. Bukelskienė, Dr. D. Baltriukienė. 2011–2012.

Project: Molecular Mechanisms of Toxicity and Antitumor Activity of Quinones and Polyphenols: Enzymatic Redox Reactions, Cytotoxicity, Signal Transduction, and Proteomics. Habil. Dr. N. Čėnas, 2011–2015. Project participants: Dr. V. Bukelskienė, Dr. D. Baltriukienė.

MAIN PUBLICATIONS

Articles

P. Danilevičius, S. Rekštytė, E. Balčiūnas, A. Kraniauskas, R. Širmenis, D. Baltriukienė, M. Malinauskas, V. Bukelskienė, R. Gadonas, V. Sirvydis, A. Piskarskas. Direct Laser Fabrication of Polymeric Implants for Cardiovascular Surgery // Materials science = Medžiagotyra / Kaunas University of Technology, Academy of Sciences of Lithuania. Kaunas : Technologija. 2012, vol. 18, no. 2, p. 145-149.

Materials of conferences

E. Stankevičius, E. Balčiūnas, M. Malinauskas, G. Račiukaitis, D. Baltriukienė, V. Bukelskienė. Holographic lithography for biomedical applications // Proceedings of SPIE. 2012, Vol. 8433 : Laser Sources and Applications / eds.: Thomas Graf, Jacob I. Mackenzie, Helena Jelenkova, John Powell, p. 843312(7 p.).

E. Balčiūnas, L. Jonušauskas, V. Valuckas, D. Baltriukienė, V. Bukelskienė, R. Gadonas, M. Malinauskas. Lithographic microfabrication of biocompatible polymers for tissue engineering and lab-on-a-chip applications // Proceedings of SPIE. 2012, vol. 8427 : Biophotonics: photonic solutions for better health care III / editors: Jurgen Popp, Wolfgang Drexler, Valery V. Tuchin, Dennis L. Matthews. ISBN 9780819491190. p. 84271X(9 p.).

OTHER SCIENTIFIC ACTIVITIES

Dr. V. Bukelskienė –

board member of European Federation for Laboratory Animal Associations, (http://www.felasa.eu/about-us/members);
board member of Baltic Laboratory Animal Science Association, (http://www.baltlasa.gf.vu.lt).

Dr. D. Baltriukienė –

President of Baltic Laboratory Animal Science Association, (http://www.baltlasa.gf.vu.lt).

COOPERATION

Laser Research Centre of the Faculty of Physics, Vilnius University (Lithuania)
Centre of Heart Surgery, Hospital Santariškių klinikos, Vilnius University (Lithuania)
Institute of Odontology, Faculty of Medicine, Vilnius University (Lithuania)
Institute of Biotechnology, Vilnius University (Lithuania)
Department of Chemistry and Bioengineering , Faculty of Fundamental Sciences, Vilnius Gediminas Technical University (Lithuania)
CC Prodentum
CC Centrinis parkas
CC Grunto valymo technologijos
CC Profarma

DEPARTMENT OF SCIENTIFIC INFORMATION

12 Mokslininkų, LT-08662 Vilnius
Tel. 272 9144, fax 272 9196
E-mail:
www http://www.bchi.vu.lt

Head –Dr. Kastis Krikštopaitis

STAFF

Senior research fellow: Dr. K. Krištopaitis.
Research fellow: Dr. G. Mikulskienė.

RESEARCH INTERESTS

Voltammetric behaviour of heterocyclic compounds
Computer-assisted molecular modelling for NMR spectral analysis

RESEARCH PROJECTS CARRIED OUT IN 2012

Projects Supported by University Budget

Synthesis, Studies of Physicochemical Properties, Enzymatic Reactions and Cytotoxicity of Quinones and Nitroheterocyclic Compounds Possessing Antiparasitic Activity. (Joint Project with the Department of Xenobiotics Biochemistry). Habil. Dr. N. Čėnas. 2011–2016.

MAIN PUBLICATIONS

Materials of conferences

R. Vaickelionienė, V. Mickevičius, G. Mikulskienė. 1-(4-Halogenfenil)-5-oksopirolidin-3-karbohidrazidų kondensacijos reakcijų produktai // Organinė chemija = Organic chemistry : mokslinės konferencijos pranešimų medžiaga. Kaunas : Technologija, 2012, p. 27-30.

U. Jagminienė, V. Mickevičius, G. Mikulskienė. 4-Fenilamino-3-(1H-benzimidazol-2-il)butano rūgšties darinių sintezė ir struktūra // Organinė chemija = Organic chemistry : mokslinės konferencijos pranešimų medžiaga. Kaunas : Technologija, 2012, p. 30-32.

OTHER SCIENTIFIC ACTIVITIES

Dr. K. Krikštopaitis –

board member of the Lithuanian Biochemical Society (LBD).