Mass spectrometers and liquid chromatographers systems for proteomics analysis (MALDI TOF/TOF, Qtrap4000, Higher Definition Mass Spectrometer Synapt G2)

Unit: Life Sciences Center

Keywords: Mass spectrometer, proteomic analysis, protein identification, de novo, sequencing, posttranslational, molecular, biochemistry, biotechnology, microbiology, biomedicine.

Responsible person: Dr. Marija Ger , tel. +370 5 223 4411

Proteomics Center at Vilnius University Institute of Biochemistry is dedicated to perform proteomics analysis starting from sample preparation by 1D and 2D electrophoresis as well as chromatography and subsequent mass spectrometry protein identification and quantitation.

Proteomics center is equip with MALDI TOF-TOF mass spectrometer used mainly for protein identification from gel samples, Q-trap 4000 hybrid triple-quadruple and linear trap mass spectrometer for protein posttranslational modification analysis and recently acquired advanced Higher Definition Mass Spectrometer Synapt G2 for label-free high throughput differential proteome analysis.

Technical characteristics
Synapt G2 HDMS mass spectrometer consists of a sample preparation component chromatograph nanoACQUITY UPLC system, Waters directly connected to the mass spectrometer and software for data analysis.

Application.

1.Protein identification by mass spectrometry;
2.Identification of protein posttranslational modifications;
3.De novo peptide sequencing;
4.Identification of protein molecular weight.