Programmable RNA shredding by the type III-A CRISPR-Cas system of Streptococcus thermophilus (I)
- Kryptis: Biotechnologijos
A Type III-A CRISPR-Cas (St Csm) complex of Streptococcus thermophilus comprising crRNA, Csm4, and Csm3 and use for cleavage of RNA bearing a nucleotide sequence complementary to the crRNA, in vitro or in vivo. Methods for site-specific cleavage/shredding of a target RNA molecule using an RNA-guided RNA endonuclease comprising a minimal complex of crRNA, Csm4, and Csm3, and methods of RNA knock-down and RNA knock-out are disclosed. 999211.
Jurisdiction: EP, US